Composite

Part:BBa_K1072007:Design

Designed by: Junjie zhang, Yichong wang, Zewei yan, Rui chen and Xuenian chen   Group: iGEM13_SCUT   (2013-09-07)


Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1970
    Illegal BsaI.rc site found at 2697
    Illegal BsaI.rc site found at 2916


Design Notes

-


Source

Some part get from registry and α-ALS gene originate from Lactococcus lactis, respectively.

References